Scholarly Article
SERUM NITRIC OXIDE METABOLITES AND ADENOSINE DEAMINASE ACTIVITY IN ASTHMA AND CHRONIC OBSTRUCTIVE PULMONARY DISEASE: A CROSS-SECTIONAL COMPARATIVE STUDY
Raut, Anita, Omkar Shete, Stish C More, Dhananjay V. Andure
2026-09-22 · International Journal of Clinical and Biomedical Research · Sumathi Publications
Abstract
Background: Nitric oxide (NO)-related metabolites and adenosine deaminase (ADA) are linked to inflammatory signalingin the respiratory tract, but circulating concentrations may differ between asthma and chronic obstructive pulmonarydisease (COPD). Objective: To compare serum NO-related metabolites and ADA activity among healthy controls, patientswith bronchial asthma, and patients with clinically stable COPD, and to examine their associations with lung function.Methods: This cross-sectional comparative study included 220 participants: 100 healthy non-smoking controls, 60 patientswith asthma, and 60 patients with COPD. Serum NO-related metabolites were estimated using a kinetic cadmium-reduction method, and serum ADA activity was measured by the Giusti-Galanti colorimetric method. Spirometry includedforced vital capacity (FVC), forced expiratory volume in one second (FEV1), and FEV1/FVC. Group differences wereassessed using one-way analysis of variance followed by Tukey post hoc comparisons, and associations with FEV1 (%predicted) were examined using Pearson correlation Results: Mean serum NO-related metabolite concentrations were 19.5± 4.2 μmol/L in controls, 32.1 ± 5.8 μmol/L in asthma, and 27.4 ± 6.1 μmol/L in COPD (overall p < 0.001). Mean ADAactivity was 12.3 ± 2.7 U/L, 22.8 ± 4.5 U/L, and 26.1 ± 5.2 U/L, respectively (overall p < 0.001). Post hoc testing indicatedsignificant differences between all three groups. In the disease groups, serum NO-related metabolites and ADA activitywere inversely correlated with FEV1 (% predicted). Conclusion: Asthma and COPD were associated with highercirculating NO-related metabolites and ADA activity than observed in healthy controls, with the highest NO-relatedmetabolite concentration in asthma and the highest ADA activity in COPD. These findings support further investigation ofthese measures as adjunctive biomarkers of inflammatory burden; however, the cross-sectional design, potentialconfounding, and use of a systemic NO surrogate preclude causal or diagnostic claims.
Keywords
Adenosine deaminase, Asthma, Chronic obstructive pulmonary disease, Nitric oxide metabolites, Nitrate, spirometry, Oxidative stress
Citation Details
International Journal of Clinical and Biomedical Research, Vol. 11, No. 3, pp. 405-410