Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Rakotonindrina, Francine Isabelle; S, Rakotomalala Rivo; A, Tsatoromila Fenosoa; Tahirimalala, Rabenandrianina; Davidra, Rajaonatahina; A, Rakotovao Luc; Olivat A, Rakoto Alson; Andry, Rasamindrakotroka; Zely, Randriamanantany; R S, Razanakolona Lala

Scholarly Article

Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Rakotonindrina, Francine Isabelle, S, Rakotomalala Rivo, A, Tsatoromila Fenosoa, Tahirimalala, Rabenandrianina, Davidra, Rajaonatahina, A, Rakotovao Luc, Olivat A, Rakoto Alson, Andry, Rasamindrakotroka, Zely, Randriamanantany, R S, Razanakolona Lala

2020-05-28 · South East Asia Journal of Medical Sciences · Little Bay Publishers

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Abstract

The aim of this study was to compare the technical performance of the Toxogen® rapid test against the ELISA technique for serological screening and/or serological monitoring of toxoplasmosis in pregnant women. Methods and material: A prospective, descriptive and analytical study was conducted over a period of 7 months from January to July 2018. One hundred and eighty-two toxoplasmosis serologies were carried out on 144 pregnant women followed in two hospitals in Mahajanga and in a private laboratory in Antananarivo. Two serologies were performed for each case, the first by the rapid test Toxogen®, a latex agglutination test, and the second by the IgG-ELISA and IgM-ELISA technique. Results: The Toxogen® rapid test had a sensitivity of 62.5%, a specificity of 97.1%, a Positive Predictive Value of 94.3%, a Negative Predictive Value of 76.7% compared to the IgG-ELISA technique. The correlation between the dilution titer by the Toxogen® rapid test and the optical density (OD) of anti-toxoplasmics IgG by the ELISA technique was very low (r² = 0.17, p <0.001). The crude prevalence of toxoplasmosis was 30,6% (n=44) by the Toxogen® rapid test and 46.5% (n=67) by the IgG-ELISA technique, the difference was significant (p <0.001). We had 2 cases (0.01%) positives by the IgM-ELISA which were not detected by the Toxogen® rapid test. Conclusion: The Toxogen® rapid test cannot substitute the ELISA technique for the serological screening and/or serological monitoring of toxoplasmosis in pregnant women. Keywords: ELISA; pregnant women; latex agglutination test; serology; toxoplasmosis.

Keywords

ELISA, pregnant women, latex agglutination test, serology, toxoplasmosis

Citation Details

South East Asia Journal of Medical Sciences, Vol. 4, No. 2, pp. 1-6