Scholarly Article
Effect of Benzalkonium Chloride as Antibacterial Activity on Dental Composites Containing Nano-Titanium dioxide Particles: in vitro study.
Atbayga A1, Abdelaaziz M2*
2020-06-01 · AlQalam Journal of Medical and Applied Sciences · University of Tripoli Alahlia
Abstract
Aim: The objective of this in vitro study was to evaluate the antimicrobial activity of resin-based dental composites containing Nano-Titanium dioxide Particles that were set with benzalkonium chloride (BzCl). Methods: TiO2 nanoparticles were treated with (3-mercaptopropyl) trimethoxysilane (MPTMS). Three groups of resin-based dental composites were prepared, namely: Group 1 (an untreated TiO2 nano-composite), Group 2 (treated TiO2 nano-composite) and Group 3 (treated TiO2 nano-composite was combined with three concentrations of benzalkonium chloride (BzCl). Five bacteria were selected and tested against the composite nano-TiO2 particles, namely, Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Enterococcus faecalis (E. faecalis), and Pseudomonas aeruginosa (P. aeruginosa), were grown on Müller-Hinton Agar (MHA), and Streptococcus mutans (S. mutans) was grown on Brain Heart Infusion (BHI) agar. Results: The results of the present study showed that neither of the groups of TiO2 nano-composites, (untreated TiO2 nano-composite and treated TiO2 nano-composite) exhibited antimicrobial activity against the pathogens. Only preparations of TiO2 nano-composites at a concentration of 3 %m/m of BzCl showed antimicrobial activity against S. aureus. Antimicrobial activity against S. mutans, E. coli, P. aeruginosa, E. faecalis and S. aureus, were only realized at a concentration of 10 %m/m for BzCl. Conclusions: Dental composites nano-TiO2 particles prepared and provided with antibacterial substances such as BzCl may prevent the growth of bacteria and may be beneficial as an additive in dental restorations.
Keywords
Titanium oxide (TiO2), Antimicrobial activity, Benzalkonium Chloride (BzCl).
Citation Details
AlQalam Journal of Medical and Applied Sciences, Vol. 3, No. 2, pp. 1-6